Accelerating phage-display library selection by reversible and site-specific biotinylation
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چکیده
منابع مشابه
Accelerating phage-display library selection by reversible and site-specific biotinylation.
Immobilization of a target molecule to a solid support is an indispensable step in phage display library sorting. Here we describe an immobilization method that addresses shortcomings of existing strategies. Our method is based on the use of a polyhistidine-tagged (His-tagged) target molecule and (BT)tris-NTA, a high-affinity capture reagent for His-tags that also contains a biotin moiety. (BT)...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2009
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzp053